Type of samples provided by user	Type of service performed	Instruments	Fees for NCMRR or MRDDRC users	Fees for external users
Crude protein extract	Full two dimensional gel electrophoresis analysis. Includes: samples desalting, IEF/PAGE, Commassie or Silver stain and image scan).   * Protein identification and gel image analyses are not included in this service.	11 cm IPG strips for first dimension and criterion format gels (10 x 15 cm) for the second dimension.	$ 90 / sample	$ 225 / sample
		17 cm IPG strips for the first dimension and large format gels (20 x 20 cm) for second dimension.	$ 180 / sample	$ 450 / sample
Crude protein extract	One dimensional gel electrophoresis (SDS-PAGE)	Criterion gels (10 x 15 cm) with 12, 18 or 26 wells.	$ 40 / gel	$ 100 / gel
Gel separated proteins or HPLC purified proteins	Enzymatic digestion and mass spectrometry analysis for protein identification. Includes: tryptic digestion, sample clean up (Zip-Tip) and protein identification.	ABI 4700 MADLI TOF-TOF. Peptide mass fingerprint.	$ 50 /spot or band	$ 125/spot or band
		Advanced MS and MS/MS analysis. ABI 4700 TOF-TOF or LC-MS/MS (LTQ).	$ 70/spot or band	$ 175/spot or band
Crude protein or peptide mixture	LC-MS and LC-MS/MS analysis. Protein expression profiling. Includes: tryptic digestion, analysis and protein identification.	Nano-HPLC-LTQ Finnigan.	$ 100/ sample	$ 250 / samples
Purified peptides or proteins	Molecular mass determination. (MS analysis only)	ABI 4700-TOF-TOF	$ 10/ sample or HPLC fraction	$ 25 per sample or HPLC fraction
	MS and MS/MS plus protein or peptide identification.	ABI 400 TOF-TOF	$ 20/ sample or HPLC fraction	$ 50 per sample or HPLC fraction

NCMRR/MRDDRC charges include reagent costs, and reflect actual costs (the Center realizes neither profit nor loss).  Consultation regarding study design, data interpretation, and publication/public access are also provided at no cost to NCMRR core users. If data analysis becomes extensive or highly specialized, it will be carried out on a collaborative basis.